TABLE 1.
Consequences of mutation of the YXXL motif on FGFR2 IIIb C1 function
|
Activity
|
FGFR2 III
C1a
|
|||
|---|---|---|---|---|
| WT | Y770F | L773A | Y770F/L773A | |
| FGFR2 transforming activityb | ± | ++ | ++ | ++++ |
| FGFR2 internalization ratec | ++ | ++ | + | + |
| FGFR2 expression levelsc | + | + | ++ | ++ |
| FGFR2 autophosphorylationb | + | + | ++ | ++ |
| PLCγ phosphorylationb | + | – | + | – |
| FRS2 phosphorylationb | ± | + | + | ++ |
| FRS2-FGFR2 bindingb | + | ++ | + | ++ |
| Phospho-MEK1/2b | – | – | – | – |
| Phospho-ERK1/2b | – | – | – | – |
| Gab1 phosphorylationb | – | – | – | – |
| Phospho-AKTb | – | – | – | – |
a
Data were compiled from this study
b
Data were determined at steady state
c
Data were transient and FGF7-stimulated