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. Author manuscript; available in PMC: 2009 Feb 27.
Published in final edited form as: J Biol Chem. 2007 Mar 16;282(21):15778–15789. doi: 10.1074/jbc.M611485200

FIGURE 3. Inhibition of D1-mediated cAMP accumulation by PSD-95.

FIGURE 3

A, PSD-95 co-expression diminishes SKF81297-induced cAMP accumulation in D1 stable HEK293 cells. PSD-95-GFP- or mock-transfected cells were treated with saline or SKF81297 (10 μM) in the presence or absence of SCH23390 (10 μM) for 30 min. cAMP accumulation was measured with an enzyme immunoassay. **, p < 0.01, Student’s t test. B, dose-response curves of cAMP production in mock- or PSD-95-GFP-transfected D1 stable cells. Cells were stimulated with increasing concentrations of SKF81297 for 30 min. The maximum cAMP accumulation (Bmax) was reduced by PSD-95 co-expression. C, unaltered competition binding curves for mock- or PSD-95-GFP-transfected D1 stable cells. D, PSD-95 co-expression did not alter IC50or the total binding determined at the saturating doses. The data represent the mean ± S.E. of three independent experiments. Competition binding experiments were carried out using 400 pM [3H]SCH23390 and nine concentrations of competing SKF81297 ranging from 1 pM to 100μM. Each data point represents the mean ± S.E. of three replications. All experiments were repeated at least three times with essentially identical results.