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. Author manuscript; available in PMC: 2009 Feb 27.
Published in final edited form as: Nat Cell Biol. 2008 May 11;10(6):654–664. doi: 10.1038/ncb1728

Figure 3.

Figure 3

The TβRI receptor is sumoylated on lysine 389. (a) Mutation of Lys389 abolishes TβRI sumoylation. 293T cells expressed Flag-tagged wild-type or mutant TβRI with the indicated lysine-to-arginine mutation, with myc-tagged SUMO-1 and Ubc9. Cell lysates were subjected to immunoprecipitations using anti-Flag, followed by western blotting for SUMO-1. (b) In vitro sumoylation of wild-type TβRI or K389R TβRI. Immunopurified TβRI was subjected to in vitro sumoylation followed by immunoblotting to detect sumoylation. (c) Proposed structure of the TβRI cytoplasmic domain. The Lys389, L45 loop, GS region and ATP binding site are indicated. N and C represent the N- and C-termini. (d) Sequence alignment of the TβRI sequence containing Lys389 and corresponding regions of other type I receptors.