Table 1.

Kinetic Parameters

		Vmax
	WT	H226Q	H434Q	H420Q
LPC 16:0 a	306 ± 60	149 ± 63	111 ± 26	359 ± 63
LPC 18:0 a	129 ± 56	76 ± 37	NS	149 ± 49
LPC 18:1 a	589 ± 96	267 ± 59 *	107 ± 13 *	472 ± 143
FS-3 a	142 ± 4	26 ± 1	NS	ND
pNpTMP b	21 ± 0.8	7 ± 2.0	NS	ND
		Km
	WT	H226Q	H434Q	H420Q
LPC 16:0 c	66 ± 11	126 ± 9 *	120 ± 15 *	94 ± 8
LPC 18:0 c	89 ± 9	270 ± 152	NS	115 ± 31
LPC 18:1 c	73 ± 8	126 ± 20	105 ± 21	69 ± 18
FS-3 c	2.6 ± 0.3	19 ± 1.1	NS	ND
pNpTMP d	0.7 ± 0.1	4.8 ± 2.2	NS	ND

^a: Units are RFU/min./μg ATX protein ± SEM.

^b: Units are A405 × 10000/min./μg ATX protein ± SEM.

^c: Units are μM ± SEM, ^d: Units are mM ± SEM.

*: p < .05 vs WT (one-way ANOVA with Dunnett's post test).

NS: data not significant, ND: not determined.