Table 4.

Summary of Methods performance

METHOD	Overall Performance Assessment
Method 1 – Affymetrix one-cycle target labeling of	Lowest sensitivity
whole blood RNA (no depletion_Affymetrix)	Good reproducibility
	Good target integrity
Method 2 – Affymetrix one-cycle target labeling of	High sensitivity
whole blood RNA with globin PNAs during cDNA	High reproducibility
synthesis (Globin PNAs_Affymetrix)	Good target integrity
Method 3 – Affymetrix one-cycle labeling of whole	High sensitivity
blood RNA treated with Ambion GLOBINclear	Lowest reproducibility
(GLOBINclear_Affymetrix)	Marginal target integrity
Method 4 – NuGEN Ovation v1 amplification and	Moderate sensitivity
labeling of whole blood RNA (no depletion_NuGEN)	High reproducibility
	Good target integrity