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The Yersinia PhoP protein binds to the ugtL promoter but cannot recruit RNA polymerase. (A–D) Promoter occupancy by PhoP (A and B) and RNA polymerase (C and D) determined by ChIP in isogenic Salmonella strains expressing the Salmonella (EG13918) or the Yersinia (EG17569) phoPQ operon. Cells were grown in N-minimal medium containing 10 mM (H) or 10 μM (L) MgCl2. Shown are the mean plus SD of at least 3 independent experiments.
