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Notch impairs goblet cell differentiation independently of Tcf4. Paraffin longitudinal sections of neonatal small intestine from wild-type (A), vilCre/Nic (B), Tcf4−/− (C), and vilCre/Nic;Tcf4−/− (D) mice stained with Alcian blue to reveal differentiated goblet cells are shown. The light blue cells evident in A and C are absent in the presence of the activated Notch transgene (B), even in a Tcf4−/− background (D). (Scale bar, 50 μm.)
