Fig. 3.

Expression of genes in the MEK/ERK output profile is down-regulated after MEK inhibition. The MEK dependence of genes identified as components of the MEK/ERK transcriptional output profile is confirmed by immunoblot and RT-PCR. (A) SkMel-5 cells in tissue culture were treated for the times indicated with 50 nM PD0325901 and lysates were subjected to immunoblotting with the indicated antibodies. (B) mRNA was isolated from SkMel-5 treated with drug as in A and analyzed by RT-PCR for levels of the indicated mRNAs. Values are relative mRNA level compared with those before treatment.