Figure 5.

CHMP1 proteins are required for efficient cytokinesis. Simultaneous depletion of CHMP1A and CHMP1B causes cells to arrest during cytokinesis and to accumulate multiple nuclei. Top, confocal immunofluorescence images are analogous to those shown in Figure 4A, except that cells in the bottom panel were treated with siRNAs targeting both CHMP1A and CHMP1B. Bottom, quantification of cells with multiple nuclei (dark bars) or with midbodies (light bars). These experiments are analogous to those shown in Figure 4C, except that 1) cells were treated with different pairs of siRNAs that targeted both CHMP1A and CHMP1B, and 2) only CHMP1A protein levels were examined because CHMP1B antibodies are not yet available.