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. 2009 Mar 1;20(5):1374–1387. doi: 10.1091/mbc.E08-05-0474

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© 2009 by The American Society for Cell Biology

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Figure 6. — hIST1 is required for cytokinesis. (A) HeLa cells were transfected with siRNA targeting CEP55, ALIX, hIST1 (hIST1-3, hIST1-4, and Dharmacon's siGENOME SMARTpool (hIST1-SP), or a luciferase control and scored for multinucleation. Representative micrographs are shown (B) in which tubulin is labeled in red. HeLa cells stably expressing YFP (−), siRNA-resistant YFP-hIST1 (WT), or siRNA-resistant YFP-hIST1 L375A/K376A were treated with the indicated siRNA and scored for multinucleation or dividing cells with intercellular bridges. Error bars indicate the SD from the mean of triplicate measurements. siRNA-mediated depletion of the indicated endogenous protein was examined by immunoblotting with α-CEP55, α-ALIX, or α-hIST1. Protein loading control was determined with α-HSP90.