Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Mar 1;20(5):1348–1359. doi: 10.1091/mbc.E08-09-0971

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 by The American Society for Cell Biology

PMC Copyright notice

Figure 2. — HSET affects spindle morphology in spindles lacking K-fibers. (A) Control HeLa cells (top three rows) or GFP-HSET overexpressing HeLa cells (bottom row) were transfected with either control Luciferase, hNuf2, or hNuf2+HSET siRNAs, synchronized, released, and then processed for immunofluorescence. In each panel, the Hec1 (magenta), HSET (green,) and DNA (blue) staining are shown. Scale bar, 10 μm. (B) Histograms of pole-to-pole distances quantified from a total of 60 spindles in three independent experiments for each knockdown condition. The best fit Gaussian distribution is shown superimposed in magenta. Average spindle lengths: control, 14.0 ± 0.4 μm; hNuf2 RNAi, 18.3 ± 0.5 μm; hNuf2 + HSET RNAi, 15.5 ± 0.6 μm; and hNuf2 RNAi + HSET overexpression, 19.7 ± 0.4 μm.