Skip to main content
. 2009 Mar 1;20(5):1348–1359. doi: 10.1091/mbc.E08-09-0971

Figure 5.

Figure 5.

Ran regulates the ability of XCTK2 to control spindle length. (A–C) GFP, GFP-XCTK2, GFP-XCTK2 NLSa, and GFP-XCTK2 NLSb proteins were added to cycled Xenopus egg extracts at a fivefold molar excess relative to the endogenous XCTK2 concentration at the time of the second CSF addition. (A) Representative fields of view showing MTs (magenta), the GFP fusion protein (green), and DNA (blue). (B) Western blot of samples from A that were probed with both anti-GFP antibodies (top panel) and anti-tubulin antibodies (bottom panel) to show that equivalent amounts of each protein were added to the extract. The GFP control concentration is below the detection limit of the antibody. (C) Quantification of the spindle length in extracts containing 10-fold excess exogenous GFP, GFP-XCTK2 (XCTK2), GFP-XCTK2 NLSa (NLSa), and GFP-XCTK2 NLSb (NLSb) proteins. A total of ∼80 structures were measured in each of three independent extracts, and the mean ± SEM is reported. Average spindle lengths: control GFP, 31.4 ± 1.7 μm; XCTK2, 34.6 ± 3.3 μm; NLSa, 69.2 ± 3.3 μm; and NLSb, 85.2 ± 11.0 μm. Scale bar, 50 μm.