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OCA2 dileucine motifs confer internal localization in melanocytes. Wild-type melan-Ink4a melanocytes were transfected with hTfR (a–c), OCA2-TfR (d–f), or AA123-TfR (g–i) and incubated at 4°C with antibodies to the extracellular region of hTfR (b, e, and h). After subsequent washing, fixation, and permeabilization, cells were labeled with NOCA2 antibody (c, f, and i) to identify transfected cells expressing chimeric proteins. Bright field images are shown in a, d, and g. Bar, 10 μm.
