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. 2009 Mar 1;20(5):1280–1288. doi: 10.1091/mbc.E08-10-1005

Figure 1.

Figure 1.

Induction of HO-1 in primary human macrophages. (A and B) Western analysis of HO-1 expression after incubations of macrophages with AC-CM (A) or MΦ-CM (B) for times as indicated. (C) HO-1 expression in macrophages treated with CM of AC, NC, or VC cells for 24 h. (D) HO-1 promoter activity in primary human macrophages after transfection of individual reporter constructs and stimulation with MΦ-CM for 18 h. Firefly luciferase activity was normalized to Renilla luciferase activity. Data are means ± SEM of at least four independent experiments. Asterisks mark statistically significant differences (p ≤ 0.05). (E) HO-1 expression in primary human macrophages after incubation with AC-CM for 6 h in the presence or absence of 5 μM SB203580. (F). Treatment of macrophages with AC-CM for 24 h with 1 μM Jak inhibitor I being present. Western blots are representative for at least three individual experiments.