Figure 7.

Proteolytic activity of Tks4 null and Tks4 wild-type Src-MEFs. (A) Conditioned media from Src-transformed Tks4^−/−, Tks4^+/+, and Tks4-GFP expressing Tks4^−/− MEFs were collected and assayed for gelatinase activity. (B) Src-transformed Tks4^−/− MEFs were plated on glass coverslips for 20 h, fixed, and stained for MT1-MMP and Tks5, and visualized by confocal microscopy. (C) Src-transformed Tks4^+/+ MEFs were plated on glass coverslips for 20 h, fixed, and stained for MT1-MMP and Tks5, and visualized by confocal microscopy.