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. 2009 Mar 1;20(5):1269–1279. doi: 10.1091/mbc.E08-08-0872

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© 2009 by The American Society for Cell Biology

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Figure 4. — Force-induced redistribution of FilGAP. Wild-type and FLNa-knock down HEK (A) and wild-type and FLNa-knockdown NIH 3T3 cells (B) were plated on fibronectin-coated glass coverslips, transfected with HA-tagged FilGAP or HA-tagged FilGAP^ΔCC for 48 h, and then either untreated or subjected to force. FilGAP was localized by staining the cells with anti-HA antibody. (C) FLNa-knockdown HEK cells were double-transfected with HA-tagged FilGAP and DsRed-FLNa construct, incubated with collagen beads, and subjected to force. (D) Double-transfection of FLNa-knockdown HEK cells with HA-tagged FilGAP and DsRed-FLNa^Δ23 constructs. Transfected cells were incubated with collagen beads and subjected to force. Cellular distribution of DsRed-FLNa was directly visualized (red), whereas FilGAP was detected with anti-HA antibody (green). Bar, 10 μm.