Table 2. List of primers used and the PCR product size.

Exon	Primer	Sequences (5'-3')	Amplicon
1	1F	AGGGAACCGTGGCTCGGC	207
	1R	GGGTGAGGGAAGTGGCTGC
2	2.1F	TTATCTCTCACTCTCCAGCC	178
	2.1R	CTGTTGTTGCTTGAAGACCAC
	2.2F	AAACTCTCACCAGCAACTCC	197
	2.2R	GGAGACCTGTCTGAATATTGC
3	3F	GGACGTATGCTGTTGAACCAC	166
	3R	TGAGCCCAAAGCAGCCACCA
4	4F	TGCAGCTGCCCGAGGATTAA	133
	4R	CCGAAGTCCCAGAAAGACCA
5	5.1F	CTCTTCTTCCTCTTCACTCTG	163
	5.1R	CGCTGTGAGCTAGCTCTAC
	5.2F	CGGTGGTGTCTTTGTCAACG	170
	5.2R	AGAGGGCGTTGAGAGTGG
5a	5aF	CTCTACAGTAAGTTCTCATACC	173
	5aR	GGAAGTGGACAGAAAACCAC
6	6.1F	TGGTTTTCTGTCCACTTCCC	167
	6.1R	GCACTCCCGCTTATACTGG
	6.2F	CCGAGAGTAGCGACTCCAG	184
	6.2R	AGGAGAGAGCATTGGGCTTA
7	7.1F	GTGAGCTGAGATGGGTGA	175
	7.1R	CTTCCGGTCTGCCCGTTC
	7.2F	ATGGGCGCAGACGGCATG	171
	7.2R	GACAGGCAAAGGGATGCAC
8	8.1F	CCCTTTTGGAGGCTCCAAG	185
	8.1R	GATGTTCTATTTCTTTGCAGC
	8.2F	TCCAACGGAGAAGATTCAG	176
	8.2R	TCTTTGTACTGAAGATGTGGC
9	9F	GGAGGTGGGAACCAGTTTGA	195
	9R	GTGAAACTGCACAGTCTCTC
10	10.1F	CTCGACGTAGACACAGTGC	175
	10.1R	AATTGGTTGGTAGACACTGG
	10.2F	TCAGAGAAGACAGGCCAGC	153
	10.2R	CCCGGAGCAAACAGGTTTAA
11	11F	GGGCTCTGACTCTCACTCTG	191
	11R	TTATGCAGGCCACCACCAGC
12	12.1F	GCTGTGTGATGTGTTCCTCA	168
	12.1R	GACTGTTCATGTGTGTCTGC
	12.2F	CTGCATGCTGCCCACCAG	163
	12.2R	AAGAGAGATCGCCTCTGTGC
13	13F	CATGTCTGTTTCTCAAAGGG	202
	13R	CCATAGTCACTGACTGAATTAACAC

For each pair of primers, the amplicon size is indicated in base pairs. F: forward primer; R: reverse primer. Exons 2, 5, 6, 7, 8, 10, and 12 were amplified in two overlapping fragments. In each case, primer pair (.1F and .1R) amplifies the most upstream 5' part of the target genomic DNA, and primer pair (.2F and .2R) amplifies the most downstream 3' part of the target genomic DNA encompassing the specific exonic sequences. Sequences are given in the 5'-3' direction.