Table 2. Antibodies used in this study and their specificities.

Primary Antibody	Specificity	Companies
I-Ad	Mouse anti-mouse MHC-II antigen	Pharmingen, San Diego, CA
CDllc	Hamster anti-mouse pi50.95. DC/LC marker	Serotec, Raleigh, NC
B7-1/CD80	Hamster anti-mouse B7-1 co-stimulatory molecule	Pharmingen, San Diego, CA
B7-2/CD86	Rat anti-mouse B7-2 co-stimulatory molecule	Pharmingen, San Diego, CA
F4/80	Rat anti-mouse F4/80 monocytes-macrophages marker	Serotec, Raleigh, NC
Mouse IgG2b	Control antibody for I-Ad (MHC-II) antigen	Pharmingen, San Diego, CA
Hamster IgG	Control antibody for CDllc. B7-1	Serotec, Raleigh, NC
Rat IgG 2b	Control antibody for F4/80. B7-2	Serotec, Raleigh, NC

Different antibodies were used to examine the immune cells of the murine cornea. All primary antibodies and OVA were conjugated with either Alexa Fluor 488 (green) or Alexa Fluor 594 (red) using the Monoclonal Antibody Labeling Kit according to the manufacturer's (Molecular Probes, Eugene, OR) instructions.