Table 3. Morphological features and distribution of positive cells in the murine cornea.
Pheuotype |
Morphology |
Proportions |
Distribution |
MHC-II+ cells or CDllc+ cells |
dendritic appearance with a small cell body but many long dendrites |
about two thirds |
distributed at the limbus, peripheral and paracentral cornea, but undetectable in the central area |
round or irregular shapes with a large cell body and a few short dendrites |
about one third |
throughout the cornea in a decreasing cell density from the limbus toward the center |
|
F4/80+ cells, B7-1+ cells or B7-2+ cells |
round, oblong or irregular in appearance, with a large cell body and a few short dendrites |
majority |
throughout the cornea with different sliapes in different areas |
dendriform in appearance |
minority |
difficult to address their distribution |
|
OVA+ cells | round, oblong or irregular shapes | all | throughout the cornea with a decreasing density from peripheral to central parts |
Morphological features and distribution of labeled cells in the murine cornea at 24 h after intravitreal injection of fluorophore-conjugated reagents were observed using confocal fluorescence microscopy. The corneas were examined at medium-power magnification (400X).