Fig. 2.

In subjects with normal spermatogenesis, numbers of apoptotic spermatocytes and Sertoli cells were determined in seminiferous tubules in non-smokers. (Fig. 1). In smokers, increased apoptosis was observed in primary spermatocytes (Fig. 2). VEGF imunopositivity in testes from non-smokers and smokers. a Immunopositive interstitial Leydig cells (non-smokers, Group 1); b Immunopositive interstitial Leydig cells (smokers, Group 2); c Immunopositive germ cells in seminiferous tubule epithelium (Group 1); d Immunopositive germ cells(GC) in seminiferous tubule epithelium (Group 2). NOTE: Arrows indicate immunopositive germ cells and arrowheads indicate immunopositive Leidig cells(LC). Magnification was determined for A, B, C and D X1000