Figure 2.

Position of the Thr30 in the L ferritin subunit crystal model and consequences of the mutation on the hydrophobicity of the N terminus. (A) Position of the Thr30 in the L ferritin crystal and details of its environment. Aminoacid residues Thr 30 is on the A helix, Trp90 is on the loop between the B and the C helices (B-C loop) and Leu 103 is on helix C. The glycosylated Asn is also indicated. The crystallographic data of L ferritin subunit from reference 18 were analyzed using the Pymol software. (B) Hydrophobicity cluster analysis of the A helix in the wild type L and the p.Thre30Ile mutant L ferritin subunits was carried out using the HCA software.²³ (http://bioserv.rpbs.jussieu.fr/RPBS/cgi-bin/Ressource.cgi?chzn_lg=fr&chzn_rsrc=HCA). The replacement of the threonine residue by isoleucine at position 30 extends the hydrophobic cluster (hydrophobic residues are indicated in green and the hydrophobic cluster is encircled).