Analysis of overexpression of AtTCP20 protein in planta. A, Quantification of AtTCP20 by western-blot analysis of protein extract from rosette leaves of 35S:TCP20 lines (lanes 1–8) and the wild type (WT). B, Phenotypes of plants overexpressing the AtTCP20 proteins. WT, The wild type; 20-1 and 20-4, two independent lines showing dwarf development and delay in the initiation of inflorescence corresponding to lines 1 and 4 analyzed by western blotting in A. On the left, plants are at the same magnification; for 20-1, magnification is 9×; for 20-4, magnification is 2.5×. [See online article for color version of this figure.]