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. 2009 Mar;149(3):1462–1477. doi: 10.1104/pp.108.126136

Figure 3.

Figure 3.

The modified protein AtTCP20∷EAR has dramatic pleiotropic effects on plant development. A, Detection of AtTCP20∷EAR and AtTCP20∷VP16 proteins by western-blot analysis of protein extracts from seedlings treated with (I) or without (NI) β-estradiol (5 μm) for 24 h. B, The seeds were directly sown on noninducible medium (a, d, e, and f) or on inducible medium (b, c, and g–i). a, Six-day-old noninduced seedling; b, 6-d-old induced seedling at 6× magnification (the inset shows a 6-d-old induced seedling at the same magnification as in a); c, 11-d-old induced seedling; d, 12-d-old noninduced hypocotyl section; e and f, 12-d-old noninduced longitudinal sections; g, 12-d-old induced hypocotyl section; h and i, 12-d-old induced longitudinal sections. Bars = 50 μm. j, Twenty-four-day-old noninduced plant; k and l, 7-d-old plants transferred on inductive medium and cultivated on this medium for 26 d; m and n, phloroglucinol staining of plantlets growing on inductive medium (n) or on noninductive medium (m).