Table II.
BiFC results for six kinase-interactor pairs identified by yeast two-hybrid screen
*, Clone length in amino acids if the complete coding sequence was not tested. **, Negative control failed. Bait in p735 interacts with pSY736 to reconstitute YFP.
| Pair No. | Bait ID (Function) | Prey ID (Function) | Split-YFP Results |
|---|---|---|---|
| 1 | Os01g32660 (OsMKK6) | Os10g38950 (OsMPK4) | Very strong |
| 2 | Os01g64970 (SAPK4) | Os01g64000 (OREB1) | Strong |
| 3 | Os05g32600 (R2) | Os06g22820 (CDK-activating kinase 1At-like) | Strong |
| 4 | Os05g45420 (SnRK1) | Os05g41220 (160–290*) (SNF1/AMPK/SnRK1 β-subunit) | Weak |
| 5 | Os03g02680 (Cdc2Os1) | Os03g05300 (CDK regulatory subunit) | **Bait alone forms YFP |
| 6 | Os01g62080 (LAMMER kinase) | Os08g41710 (61–160*) (FHA domain-containing protein) | No interaction detected |