Figure 5.

PUX2 T-DNA insertion mutants exhibit reduced PM reproduction. A, Macroscopic phenotype. Wild-type (Col-0), pux2-1 (SALK_148507), and pux2-2 (SALK_145175) UI whole plants were photographed at 6 weeks. G. orontii-infected leaves were rated and photographed at 11 dpi. Number of plants with disease rating is indicated as described by Reuber et al. (1998) as follows: 0, no visible symptoms of infection; 4, approximately 100% coverage of infected leaves. Bars = 1 cm. B, Microscopic phenotype. Leaves were harvested at 5 dpi and stained with trypan blue for visualization of fungus or cell death. c, Initial germinated conidia; cp, conidiophore. Bars = 100 μm. Independent experiments showed similar results.