Figure 2.

Analysis of the TYRAAt1 transcript. A, Schematic representation of the intron/exon organization of the TYRAAt1 gene. Position of primers used in this study is shown. B, Northern-blot analysis of TYRAAt1 transcript in young Arabidopsis rosette leaves. Total RNAs were isolated from young Arabidopsis rosette leaves using the RNeasy plant mini kit isolation system according to the manufacturer's instructions (Qiagen). Total RNAs (10 μg) were denatured for 1 h at 50°C in 10 mm NaH₂PO₄ (pH 7), 2% (v/v) dimethyl sulfoxide, 1.08 m glyoxal, and separated by 1% (w/v) agarose gel electrophoresis. It was then transferred to nylon membrane (Nitran). The resulting blot was subjected to hybridization with the corresponding ³²P-labeled DNA probes matching exon 3 of the Arabidopsis TYRAAt1 gene. A single transcript of 1.9 kb corresponding to the complete TYRAAt1 mRNA was hybridized.