Figure 3.

TYRA activity (A) and western-blot analysis (B) of Arabidopsis protein soluble extract separated on a S200 gel filtration column. A, Total soluble extracts were obtained from Arabidopsis plants as described in “Materials and Methods” and separated on a S200 gel filtration column. TYRA activity was determined using 300 μm arogenate and 1 mm NADP⁺ in 50 mm Tris-HCl, pH 7.5. B, Fifty micrograms of protein extract resulting from the gel filtration column was separated by SDS-PAGE using 12% acrylamide gels, transferred to nitrocellulose membranes, and probed with the antibodies raised against TYRAAt2 (left) or TYRAAt1 (right). Molecular masses are indicated on the right (in kD).