Figure 6.

Subcellular localization of the six isoforms of Arabidopsis ADT. A, Transient expression of AtADT1 to AtADT6 fused to GFP in Arabidopsis protoplasts. Constructs encoding GFP fused to AtADT1 to AtADT6 (AtADT1–6-GFP) were introduced into Arabidopsis protoplasts. Images are optical photomicrographs (Bright Field), chlorophyll fluorescence (Chloro.; red pseudocolor), and GFP fluorescence (GFP; green pseudocolor). B, Immunolocalization of ADT in Arabidopsis. Proteins, 100 μg for total soluble extract of rosette leaves (T), 100 μg for the cytosolic-enriched fraction (Cy), and 100 μg for the chloroplast stroma (Str), were separated by SDS-PAGE and analyzed by western blotting using affinity-purified antibodies raised against AtADT1.