Pie chart representing the tobacco stigma/style gene function categories. The “No hits” category is composed of the clusters/transcripts/genes that had no significant similar sequences (cutoff e-value of ≤10−5) in the NCBI nonredundant protein database after BLASTX analysis. The remaining sequences were automatically categorized by the KOGnitor tool, with a curator revision of the clusters initially categorized as “No related KOG.” The category “Function unknown” includes the clusters that matched to unknown proteins, expressed, unnamed, putative, or hypothetical proteins that were initially categorized as “No related KOG,” as well as those categorized by KOGnitor as “General function prediction only” and “Function unknown,” with no indication of their corresponding function. The slices indicate the number of clusters assigned to each category as a percentage of the total number of clusters (6,177).