Table VII.
Primers and fluorescent probes for the putative stigma/style-specific genes
| TOBEST Identifier | Primer 1 (5′–3′) | Primer 2a (5′–3′) |
|---|---|---|
| C022C03 | CATTTGCACAAGCACCACCA | caacagtTTTCCAGCACGTTCTGACTGtTG |
| C023B06 | AATTCTGCTGCTGGCTTTACCTT | catctgaTCTTCCTGGGAATGATTCAGAtG |
| C092C05 | TTGCGTTGCTTGTTCTCCCTTT | cggtgTCCCTCACTTTAATTGCACcG |
| C130F10 | GCGAGTCGCAACAACTTGG | gacttgaATGGCGAATGAGGAGATCAAGtC |
| S008H02 | TGGGAGACAGCATACCAATTTCA | cgacaGGGGTACGAATCTTGTGTcG |
| S010A10 | TCCATTTCTTGCTGGCGTTT | cacgatTTTCTTCCATCAGCATTATCGtG |
| S042D12 | GGAAACAAGGGAAGCAAACCTG | cgctaTGAGAGCAATGGGTTGTAGcG |
| S049F11 | GCATCGAGGATGTAAGGGATG | cactggGCATGACAATAAATCAGCCAGtG |
| S053D05 | CAGGATGCAATATCTGCTGTGG | cggtgCCCAAACTCCAGCTTCACcG |
| S118H02 | GAGGAAGCTCCCATTGCACTAA | cactgtatCTTGAACAGCCTGAGCATACAGtG |
a
Primers labeled with 6-carboxy-fluorescein, incorporated at the boldface, lowercase, underlined nucleotides. Lowercase letters (at the 5′ end) represent the nucleotides added to the sequence according to the LUX system for real-time PCR.