Figure 3.

Comparison of in vitro estimates of total leaf DMADP pool sizes in light and in darkness (A) and during light and after feeding for 45 to 50 min with the chloroplastic MEP pathway inhibitor fosmidomycin (20 μm) through the leaf petiole (B) in aspen. The DMADP pool in the light was estimated after steady-sate isoprene emission rates were achieved, while the dark pool was estimated when isoprene emission reached zero. n = 62 (31 pairs of leaves measured in light and in darkness) for A and n = 5 for B. The chemical estimation of total leaf DMADP pool size follows Fisher et al. (2001). Error bars denote se.