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Anucleate cell formation in muk mutant cells. Cells were grown in LB broth at 23°C. (A) DBP924 wild-type. (B) DPB923, topA10. (C) CC4208, ΔmukB∷kan. (D) CC4207, topA10 ΔmukB∷kan. (E) CC4218, mukE∷kan. (F) CC4217, topA10 mukE∷kan mutant cells. White arrows highlight some anucleate cells. Black arrows highlight filamentous cells with “DNA-free bays.”
