Table I.
Putative in vivo sumoylation substrates of Arabidopsis
Criteria for inclusion of a protein were robust detection in preparations, absence from control preparations, and apparent molecular mass, as detailed in the text. n.t., Not tested.
| Identifier | Characteristics | ΨKxD/Ea | In Vivo Conjugate to: | In Vitro Sumoylationb |
|---|---|---|---|---|
| RNA-dependent processes | ||||
| At1g29400 | AML5; has one RRM2 domain, two RRM1 domains | 1 (1) | SUM3 | Yes |
| At3g56860 | Contains two RRM1 domains | 1 (0) | SUM1 | Yes |
| At2g43970 | Contains an LA domain | 0 (1) | SUM1 | No |
| At2g47020 | Translation release factor | 1 (2) | SUM3 | n.t. |
| At3g60240 | eIF4G | 4 (2) | SUM3 | n.t. |
| Chromatin-related processes | ||||
| At1g55300 | TAF7 (TFIID subunit) | 0 (0) | SUM3, -5 | Yes |
| At2g19480 | Nucleosome assembly factor | 2 (0) | SUM1 | Yes |
| At5g08450 | Contains an Rxt3 domain | 3 (10) | SUM3 | Yes |
| At5g39660 | zfDOF transcription factor | 1 (1) | SUM1 | n.t. |
| At5g43130 | TFIID subunit | 2 (4) | SUM1 | n.t. |
| Others | ||||
| At1g27430 | Contains a GYF domain | 1 (4) | SUM1, -3 | n.t. |
| At1g64330 | Contains a KIP domain, coiled-coil region | 2 (1) | SUM1, -3 | n.t. |
| At5g13480 | FY; WD40 repeat protein | 0 (0) | SUM1, -3 | n.t. |
| At5g52300 | RD29B desiccation induced | 3 (2) | SUM1 | n.t. |
a
In addition to canonical SUMO attachment sites (Ψ is an aliphatic hydrophobic residue, x indicates any residue, D/E is either Asp or Glu), many proteins contain nearly canonical sequences as further potential SUMO attachment sites (number of such sites is given in parentheses; www.abgent.com/doc/sumoplot).
b
The in vitro sumoylation reactions referred to here used SUMO1 and its variant SUMO1 Q90A with identical results.