Table I.
Summary of bisulfite sequencing analysis of the 35S promoter within the silencing locus (Lo1) in Lo1/Lo2 parental plant, derived calli, and a regenerated plant
| Sample/Methylation Motif | mCG
|
mCHG
|
mCHH
|
|||
|---|---|---|---|---|---|---|
| Meana | Rangeb | Mean | Range | Mean | Range | |
| % | ||||||
| Parental PTGS Lo1/Lo2 plant | 1 | 0–8 | 0 | 0–0 | 1 | 0–1 |
| Disc of leaf (green tissue) | 0 | 0–0 | 2 | 0–14 | 0 | 0–3 |
| Early callus (4 weeks) | 0 | 0–0 | 0 | 0–0 | 5 | 0–11 |
| Advanced callus (12 months) | 48 | 0–83 | 51 | 0–88 | 34 | 22–44 |
| Nonsilenced regenerant (no. 2) | 90 | 83–100 | 75 | 57–100 | 34 | 22–44 |
a
Percentage methylation at CG, CHG, and CHH contexts was calculated from the clones (Fig. 6A) and expressed as a mean.
b
Methylation homogeneity between the clones is indicated by a range.