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Simultaneous intracellular and extracellular (apoplastic) recordings of SPs triggered by Ca2+ on distant leaves of H. vulgare or V. faba. Intracellular measurements were carried out on mesophyll cells while the apoplastic voltage electrode was positioned in a neighboring stoma. Kinetics representative of four equivalent tests each are shown. Em, Membrane potential; E(apo), apoplastic voltage.
