Table I.

Summary of differentially emitted volatiles and their corresponding regulatory patterns

N. attenuata leaf volatiles were collected at 1 to 13 h, 13 to 25 h, and 25 to 37 h after elicitation, and those differentially emitted (ANOVA, P < 0.05, n = 6) between the groups of treatments were analyzed by PCA (Figs. 6 and 7). Types I.a to I.c and type II contain leaf volatiles that were induced after W and induced or repressed after application of M. sexta OS. Amplification of the wound (W) response was mediated by the action of FACs (type I.a), the action of FACs or 2-HOT (type I.b), or the action of unknown factors (type I.c). Type II represents volatiles induced after W that were repressed after the application of M. sexta OS mainly through the action of FACs. Type III represents W-induced volatiles for which no significant effect of the application of OS was observed. Volatiles are listed by chemical classes with their RTs on the first and second dimensions. Numbers indicate the type of identification (Id.): 1, comparison with standards; 2, putative identification. Retention indices were calculated according to Kováts (1958) and compared with literature (Lit.) reports: ^a (Ruther, 2000), ^b (Adams, 2004), ^c (Engel et al., 2002), ^d (Triqui and Reineccius, 1995), ^e (Guth and Grosch, 1991), and ^f (Jirovetz et al., 2002).

Class	Compound	Id.	RT(s)		Retention Index		Regulation (Type)
			RT1	RT2	Calculated	Lit.	1, 1–13 h	2, 13–25 h	3, 25–37 h
Acid	Pentanoic acid, 3-methyl-	2	450	2.49	963	–	I.c		I.c
Alcohol	2-Pentanol,4-methyl	2	216	2.02	837	–	II
	2-Pentanol,3-methyl	2	228	2.17	845	–	II
	cis-3-Hexenol	1	246	2.57	858	849a	II
	1-Hexanol	1	270	2.56	872	876a	II
Aldehyde	Heptanal	2	336	3.01	910	903c,d			I.a
Aromatic	Octanal	2	552	2.73	1,004	1,002c,d	I.c
	Unknown	–	564	5.23	1,012	–	I.a	I.a
	Unknown	–	858	4.03	1,168	–	I.c	I.c
	Inden-2-one, octahydro	2	954	4.13	1,215	–	I.c
	Benzene, 2,4-diethyl-1-methyl-	2	822	2.58	1,144	–	II	II
	Benzothiazole	2	960	5.51	1,220	1,221f	II	II
Hexenylester	cis-3-Hexenylisobutyrate	1	834	2.51	1,151	1,147b	I.c	I.c	I.c
	cis-3-Hexenylpropionate	1	750	2.73	1,104	1,100a	I.c	I.c
	cis-3-Hexenyl,trans-2-butenoic ester	1	882	2.71	1,176	–	I.c	II
	cis-3-Hexenyl-3-methylbutyrate	1	996	2.51	1,241	1,245b	I.c
	cis-3-Hexenylmethylpentanoate	1	1,170	2.51	1,348	–	I.c
	cis-3-Hexenyltigilate	1	1,140	2.85	1,328	1,322a	III	II
	cis-3-Hexenylbutyrate	1	912	2.56	1,191	1,187a	III
	cis-3-Hexenylacetate	1	570	2.84	1,014	1,008a	III
	Hexylisobutyrate	1	840	2.32	1,154	1,152b	III
	trans-2-Hexenylbutyrate	1	924	2.65	1,197	1,198a	III
	cis-3-Hexenyl-2-methylbutyrate	1	984	2.51	1,234	1,231a	III
Methylester	Pentanoic, 3-methyl-, methyl ester	2	300	2.80	888	–	I.c
Terpenoid	Unidentified sesquiterpene	–	1,176	3.27	1,352	–	I.a	I.a	I.a
	Unidentified monoterpene	–	714	2.71	1,090	–	I.a		I.a
	Unidentified monoterpene	–	738	2.84	1,107	–	I.a	I.a
	1-Cyclopentylcyclopentene	2	648	2.56	1,054	–	I.a
	Unknown sesquiterpene	–	720	2.34	1,088	–	I.a
	trans-α-Bergamotene	1	1,302	2.28	1,435	1,435b		I.b	I.b
	β-Pinene	1	474	2.29	396	979c	I.c
	Unknown	–	1,476	2.88	1,541	–	II
	Terpineol	1	906	3.02	1,188	1,189b		II
	Limonene	1	600	2.46	1,030	1,031b			II
Unknown	–	–	312	4.47	897	–	I.a		I.a
	–	–	306	4.22	895	–	I.a
	–	–	612	3.51	1,036	–	I.a
	–	–	756	2.45	1,110	–	I.a
	–	–	1,056	3.56	1,287	–	I.a
	–	–	1,074	3.49	1,305	–	I.a
	–	–	522	5.44	984	–	I.b
	–	–	354	3.24	348	–	I.c
	–	–	594	2.66	1,026	–	III	III