Figure 5.

RNA degradation and DNA methylation driven by PPV is RDR6 dependent. A, Accumulation of GFP mRNA, PPV:GFP gRNA, and asGFP siRNAs in GFP and GFP/rdr6i plants systemically infected with PPV:00 or PPV:GFP (28 dpi) assessed by northern-blot analyses using probes specific for GFP and the Nopaline Synthase 3′ untranslated region of the transgenic GFP mRNA. Ethidium bromide-stained rRNAs are shown as loading controls. B, Southern-blot analysis of DNA samples extracted from the same tissue used for the RNA analysis. DNAs were digested with Sau96I digestion and analyzed using a GF-specific probe (see Fig. 2C).