Figure 2.

Wild-type and dmi3-1 epidermal cell organization upon fungal entry. Identical fluorescent ER labeling and color coding are as in Figure 1. A, G. margarita intracellular hyphae (Ih) inside two adjacent epidermal cells, about 12 h after hyphopodium adhesion. The plant nucleus (*) of the lower cell is still in the vicinity of the transcellular tunnel hosting the fungus, but the dense ER accumulation characterizing the PPA has disappeared and loose ER cisternae surround the hyphae. B, About 48 h after inoculation with C. trifolii, an intracellular vesicle (white dot) is visible inside an epidermal cell from wild-type roots (see also Supplemental Fig. S1). The vesicle is surrounded by a dense accumulation of ER and in close vicinity to the nucleus (*). C, The entry of a C. trifolii hypha into the lumen of a dmi3-1 epidermal cell also occurs at about 48 hpi in the vicinity of the nucleus (*), but is associated with the disruption of the ER, which appears broken into clusters of roughly spherical bodies containing GFP. h, Hypha. Bars = 10 μm.