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. 2009 Mar;149(3):1289–1301. doi: 10.1104/pp.108.130252

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Copyright © 2009, American Society of Plant Biologists

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Figure 6. — Pollen viability and germination in +/spp-2 plants compared with wild-type plants. A and B, Alexander-stained anthers from Col-0 wild-type (A) and +/spp-2 (B) plants. C and D, Micrographs of pollen germination assays of wild-type (C) and spp-2 heterozygote (D) pollen on detached wild-type pistils. Examples of germinated (g), polarized (p), and ungerminated (u) pollen grains are indicated. E, Proportion of pollen grains that germinated after 2 h at room temperature for wild-type (wt) siblings (n = 420; gray bars) and heterozygotes (n = 330; black bars) of spp-2. Ungerminated pollen was subdivided into polarized and nonpolarized.