Table I.

Segregation of the spp-2 mutant allele in the progeny of self-crossed and reciprocal outcrossed plants

Basta^R, Basta resistant; Basta^S, basta sensitive; wt, wild type.

Self-Crosses	No. of Progeny	Genotypes of Progeny (PCR)
		+/+ (%)	+/spp-2 (%)	spp-2/spp-2
Parental genotype
+/spp-2	81	44 (54.3)a	37 (45.7)a	0
		Resistance to Basta
		BastaS (%)		BastaR (%)
+/spp-2	643	306 (47.6)b		337 (52.4)b
Reciprocal Outcrosses	No. of Progeny	Resistance to Basta		Transmission Efficiencyc
		BastaS (+/+)	BastaR (+/spp-2)
Recipient × donor
wt × +/spp-2	376	370	6	1.6
+/spp-2 × wt	123	63	60	95.2

^aSignificantly different from the expected 1:2:1 ratio for normal Mendelian segregation (χ² = 49.2; P < 0.001). Not significantly different from the 1:1:0 ratio for a gametophytic defect (χ² = 0.605; P > 0.05).

^bSignificantly different from the expected 1:2:1 ratio for normal Mendelian segregation (χ² = 174.0; P < 0.001). Not significantly different from the 1:1:0 ratio for a gametophytic defect (χ² = 0.222; P > 0.05).

^cTransmission efficiency = (mutant/wild type) × 100 (%).