Table I.
Transcriptional changes induced by auxin treatment and IAAnmII overexpression
The number of genes with statistically significant differences (see “Materials and Methods”) in expression between all vectors and treatments are given. The average fold change in expression of the 809 genes responsive to auxin treatment in the pMON999_mRFP control vector is presented for protoplasts transformed with the control vector as well as protoplasts transformed with the IAA7mII and IAA19mII overexpressors.
| Comparison of Treatments | No. of Genes with Significant Changes in Expression
|
Average Fold Change in Expression of pMON999_mRFP Auxin-Responsive Genes | ||||||
|---|---|---|---|---|---|---|---|---|
| pMON999_mRFP
|
pBeaconRFP_IAA7mII
|
pBeaconRFP_IAA19mII
|
||||||
| Mock | IAA | Mock | IAA | Mock | IAA | |||
| pMON999_mRFP | Mock | 0 | 809 | 3,663 | 3,859 | 4,036 | 4,340 | 2.7 ± 0.2a |
| IAA | 0 | 4,182 | 4,231 | 4,513 | 4,677 | |||
| pBeaconRFP_IAA7mII | Mock | 0 | 0 | 2,139 | 2,369 | 1.6 ± 0.1ab | ||
| IAA | 0 | 2,300 | 2,378 | |||||
| pBeaconRFP_IAA19mII | Mock | 0 | 620 | 1.6 ± 0.2ab | ||||
| IAA | 0 | |||||||
a
Average values are given ± se (n = 809).
b
Statistically significant difference compared with pMON999_mRFP as determined by a two-tailed unpaired t test (P < 10−4).