Table 3.
The CHS promoters are hyper-stimulated by combined Ca2+/CFW treatment.
| Strain | Treatment | Mean % /acZ activity |
Fold changea | P-value | Fold changeb |
P-value | |
|---|---|---|---|---|---|---|---|
| CHS1p-lacZ | |||||||
| wt | Control | 100 ± 7 | |||||
| +Ca2+ | 174 ± 38 | 1.74 | 1.60E-05 | ||||
| +CFW | 190 ± 34 | 1.90 | 3.71E-02 | ||||
| +Ca/CFW | 888 ± 59 | 8.88 | 1.30E-06 | ||||
| mkc1Δ | Control | 91 ± 9 | 0.91 | 7.06E-01 | |||
| +Ca2+ | 189 ± 25 | 2.09 | 4.00E-04 | 1.20 | 1.00E+00 | ||
| +CFW | 141 ± 41 | 1.55 | 2.27E-01 | 0.82 | 7.08E-01 | ||
| +Ca2+/CFW | 735 ± 40 | 8.11 | 4.40E-10 | 0.91 | 3.08E-01 | ||
| crz1Δ | Control | 209 ± 11 | 2.09 | 3.10E-10 | |||
| +Ca2+ | 202 ± 22 | 0.97 | 1.00E+00 | 0.55 | 7.81E-01 | ||
| +CFW | 154 ± 19 | 0.74 | 3.70E-04 | 0.39 | 7.91E-01 | ||
| +Ca2+/CFW | 411 ± 174 | 1.96 | 2.78E-01 | 0.22 | 1.43E-03 | ||
| hog1Δ | Control | 39 ± 12 | 0.39 | 2.00E-06 | |||
| +Ca2+ | 48 ± 9 | 1.22 | 9.94E-01 | 0.70 | 1.44E-09 | ||
| +CFW | 47 ± 13 | 1.18 | 1.00E+00 | 0.62 | 2.80E-03 | ||
| +Ca2+/CFW | 592 ± 219 | 15.00 | 8.79E-03 | 1.69 | 3.85E-01 | ||
| CHS2p-lacZ | |||||||
| wt | Control | 100 ± 3 | |||||
| +Ca2+ | 226 ± 43 | 2.26 | 1.52E-07 | ||||
| +CFW | 221 ± 23 | 2.21 | 7.67E-08 | ||||
| +Ca2+/CFW | 889 ± 121 | 8.89 | 3.05E-06 | ||||
| mkc1Δ | Control | 68 ± 3 | 0.68 | 9.98E-11 | |||
| +Ca2+ | 196 ± 13 | 2.88 | 1.77E-08 | 1.27 | 6.02E-01 | ||
| +CFW | 76 ± 3 | 1.12 | 7.00E-03 | 0.51 | 1.08E-08 | ||
| +Ca2+/CFW | 379 ± 18 | 5.56 | 5.96E-10 | 0.63 | 5.20E-11 | ||
| crz1Δ | Control | 142 ± 10 | 1.42 | 3.80E-05 | |||
| +Ca2+ | 153 ± 12 | 1.08 | 8.91E-01 | 0.48 | 2.13E-04 | ||
| +CFW | 147 ± 6 | 1.04 | 1.00E+00 | 0.47 | 9.56E-06 | ||
| +Ca2+/CFW | 402 ± 43 | 2.84 | 2.13E-06 | 0.32 | 1.06E-05 | ||
| hog1Δ | Control | 43 ± 3 | 0.43 | 2.30E-14 | |||
| +Ca2+ | 54 ± 2 | 1.24 | 8.95E-06 | 0.55 | 1.58E-09 | ||
| +CFW | 38 ± 1 | 0.88 | 8.63E-03 | 0.40 | 1.40E-09 | ||
| +Ca2+/CFW | 354 ± 113 | 8.15 | 2.00E-03 | 0.92 | 2.67E-01 | ||
| CHS3p-lacZ | |||||||
| wt | Control | 100 ± 7 | |||||
| +Ca2+ | 154 ± 26 | 1.54 | 1.69E-04 | ||||
| +CFW | 118 ± 10 | 1.18 | 1.36E-02 | ||||
| +Ca2+/CFW | 693 ± 33 | 6.93 | 2.94E-10 | ||||
| mkc1Δ | Control | 101 ± 8 | 1.01 | 1.00E+00 | |||
| +Ca2+ | 184 ± 23 | 1.82 | 1.20E-04 | 1.18 | 4.27E-01 | ||
| +CFW | 104 ± 25 | 1.03 | 1.00E+00 | 0.87 | 9.94E-01 | ||
| +Ca2+/CFW | 732 ± 93 | 7.23 | 2.08E-06 | 1.04 | 1.00E+00 | ||
| crz1Δ | Control | 110 ± 8 | 1.10 | 4.45E-01 | |||
| +Ca2+ | 89 ± 6 | 0.81 | 8.87E-02 | 0.52 | 1.20E-04 | ||
| +CFW | 91 ± 4 | 0.83 | 2.32E-01 | 0.70 | 3.00E-01 | ||
| +Ca2+/CFW | 288 ± 41 | 2.61 | 1.66E-04 | 0.38 | 6.10E-10 | ||
| hog1Δ | Control | 156 ± 14 | 1.56 | 1.29E-05 | |||
| +Ca2+ | 251 ± 21 | 1.61 | 2.06E-07 | 1.04 | 6.24E-07 | ||
| +CFW | 135 ± 9 | 0.86 | 2.12E-05 | 0.73 | 5.15E-02 | ||
| +Ca2+/CFW | 988 ± 237 | 6.32 | 2.16E-04 | 0.91 | 2.12E-01 | ||
| CHS8p-lacZ | |||||||
| wt | Control | 100 ± 9 | |||||
| +Ca2+ | 158 ± 16 | 1.58 | 2.28E-11 | ||||
| +CFW | 263 ± 14 | 2.63 | 8.27E-07 | ||||
| +Ca2+/CFW | 1237 ± 421 | 12.37 | 9.01E-09 | ||||
| mkc1Δ | Control | 27 ± 5 | 0.27 | 1.75E-09 | |||
| +Ca2+ | 76 ± 17 | 2.86 | 7.52E-04 | 1.81 | 1.61E-01 | ||
| +CFW | 16 ± 2 | 0.59 | 6.41E-03 | 0.22 | 4.38E-08 | ||
| +Ca2+/CFW | 378 ± 47 | 14.12 | 8.50E-07 | 1.14 | 3.81E-06 | ||
| crz1Δ | Control | 141 ± 8 | 1.41 | 1.99E-06 | |||
| +Ca2+ | 160 ± 12 | 1.13 | 1.13E-01 | 0.72 | 3.95E-07 | ||
| +CFW | 233 ± 16 | 1.65 | 4.08E-07 | 0.63 | 4.13E-09 | ||
| +Ca2+/CFW | 529 ± 94 | 3.74 | 9.74E-05 | 0.30 | 4.42E-05 | ||
| hog1Δ | Control | 43 ± 8 | 0.43 | 2.03E-08 | |||
| +Ca2+ | 48 ± 5 | 1.11 | 9.99E-01 | 0.70 | 1.09E-07 | ||
| +CFW | 34 ± 6 | 0.79 | 6.00E-01 | 0.30 | 3.70E-09 | ||
| +Ca2+/CFW | 315 ± 69 | 7.29 | 1.23E-04 | 0.59 | 7.10E-04 | ||
a
Fold change with respect to the control in the same genetic background.
b
Ratio of fold change in the mutant compared with fold change in wild-type cells under the same conditions.
Statistically significant changes are highlighted in bold.