Figure 7.

Treatment of S isolate infected mice with anti-GITR does not affect IL-10 or Th1 associated cytokines but does increase some Th2 cytokines. S isolate infected C57BL/6 mice were treated i.p with 1mg anti-GITR or PBS weekly from d-7 excluding d0. At day 21 p.i MLN cells from naïve, PBS control and anti-GITR treated S isolate infected mice were isolated and cultured with parasite specific ES antigen for 48hrs. Supernatant levels of IFN-γ, TNF-α, IL-10, IL-6, IL-4, IL-5, IL-9 and IL-13 were measured by CBA; all cytokine data are pg/ml (A). Values represent the mean ± SD for five mice per group. *, significant difference between the PBS control and anti-GITR S isolate infected mouse (Mann Whitney U test, p<0.05).

Worm numbers in mice from both PBS control and anti-GITR treated groups were analysed at days 21 and 28p.i. (B) Each symbol represents an individual animal (5 mice per group) and the line represents the mean. Experiments were performed at least twice and data shown is a representative experiment.