Skip to main content
. 2009 Mar 9;4(3):e4751. doi: 10.1371/journal.pone.0004751

Figure 9. Quantitative real time RT-PCR analysis of transcriptional levels of Tribolium MMPs, TIMP, and RECK, along with immune-inducible thaumatin during innate immune responses.

Figure 9

The mRNA levels of MMP-1, TIMP, and RECK were significantly induced 2 to 5-fold in hemocytes in response to immune-challenge along with the immune-inducible thaumatin gene. In contrast, in whole animal preparations containing mainly fat body tissue no significantly induced expression of tested genes could be observed except immune-inducible thaumatin, although a tendency towards MMP-1 induction was detected. 18S rRNA gene expression was not influenced by treatment, and expression levels of MMP-2 or MMP-3 were not significantly different upon immune-challenge. Results represent mean values of three independent determinations±S.D. from ten animals that were pooled for analysis. Statistically significant differences were determined using Student's t-test and are indicated (*, p<0.05; **, p<0.01).