Figure 10. Detection of MMP-like collagenolytic activity on the surface of stimulated Tribolium hemocytes.

Typical granular hemocytes from Tribolium (A,C) exhibited MMP-like enzymatic activity localized at their surface by hydrolyzing DQ™ collagen type IV resulting in green fluorescence (B) and of quenched, fluorogenic MMP-1/MMP-9 peptidic substrate resulting in bright blue fluorescence (D), respectively. Scale bar, 10 µm.