Quantitative real time RT-PCR primer
|
RT-18S rRNA |
ATGGTTGCAAAGCTGAAACT
|
TCCCGTGTTGAGTCAAATTA
|
RT-MMP-1 |
CGATAGGGCCTTTGCTGTGG
|
CACCCCCTGGACTCACTCGT
|
RT-MMP-2 |
GCGATGTGTGTGTGCTGTGG
|
CGTTGCACCGAATCATCGAC
|
RT-MMP-3 |
GTGGGTTGGACGGGAATGAG
|
GGCGCCTCCTCCGCTATATT
|
RT-MMP-4 |
CGTTGTGGATTGCCTGATGC
|
CACATGTGCACGCCACGTTA
|
RT-TIMP |
GGCAGTAACGGTGGCGTACC
|
TCACGAAGTCGGCTCTGCAA
|
RT-RECK |
ACAGCTGCCGAAAAGCTTGC
|
GGCGTTCTAAGCAGCGGAAA
|
RT-Thaumatin |
GGCAACGGGGTTATTGCTTG
|
ACGTGTCAGGTGTGCCGAAA
|
Primers used for RNAi experiments
|
RNAi-MMP-1 |
TAATACGACTCACTATAGGGAGATCCAGAGTTTTGCCGGTCTC
|
TAATACGACTCACTATAGGGAGATTGGAAGTCCGAATCGTCGT
|
RNAi-MMP-2 |
TAATACGACTCACTATAGGGAGACGGGATTTGATGGGAATGGA
|
TAATACGACTCACTATAGGGAGATTTGTCGTCGTCGGGGAGTT
|
RNAi-MMP-3 |
TAATACGACTCACTATAGGGAGACTCCGAAGGGTGGGGAAAAC
|
TAATACGACTCACTATAGGGAGACGTCAAAGGAGGCGTCACAA
|
RNAi-MMP-4 |
TAATACGACTCACTATAGGGAGACGTTGTGGATTGCCTGATGCT
|
TAATACGACTCACTATAGGGAGAGGGATTTTTGCGTTGTTGTTGTTG
|
RNAi-TIMP |
TAATACGACTCACTATAGGGAGATGACCGCATCTGACGAAGCA
|
TAATACGACTCACTATAGGGAGA
|
|
|
TCCGAACCACCGAGAAATGG
|
RNAi-RECK |
TAATACGACTCACTATAGGGAGAATTGCCACAACCTGCCCAAA
|
TAATACGACTCACTATAGGGAGA
|
|
|
TCGTTTGCACCGTCACGAAA
|
RNAi-Dpp |
TAATACGACTCACTATAGGGAGATCGACACTGTTGCCCCTTTT
|
TAATACGACTCACTATAGGGAGATGGTTGGTTTTGGGGTCTTG
|
RNAi-IMPI (control) |
TAATACGACTCACTATAGGGAGACGGTGGAGCCTGCGATAATG
|
TAATACGACTCACTATAGGGAGACGACGGTGGAGGGGAGTCAA
|