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. 2009 Mar 9;4(3):e4756. doi: 10.1371/journal.pone.0004756

Figure 1. Silencing of PINK1 mRNA and protein expression in SH-SY5Y cells.

Figure 1

A, SH-SY5Y cells were transfected with PINK1 siRNA (PINK1 #1 or PINK1 #2) or scrambled control siRNA for 6 or 12 days and PINK1 mRNA levels measured by reverse transciption and quantitative real-time PCR. Relative expression was determined against GAPDH mRNA levels and data were expressed as mean±s.e.m. (n = 4). **P<0.01 vs. scrambled control siRNA. B, SH-SY5Y cells over-expressing recombinant PINK1 protein were transfected with either PINK1 siRNA (PINK1 #1 or PINK1 #2) or scrambled control siRNA for 40 hours. The expression of full-length recombinant PINK1 protein was determined in cell lysates by western blotting. No band was detected in SH-SY5Y transfected with empty vector. Protein loading was verified with antibody against succinate dehydrogenase subunit SDHA.