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. 2009 Mar 9;4(3):e4755. doi: 10.1371/journal.pone.0004755

Figure 1. LT destroys barrier function in differentiated mucociliary human lung epithelium.

Figure 1

(A–D) Polarized NHBE were treated with or without LT for 48 h. (A) The epithelial layer was subjected to resistance measurements (left panel) or incubated with FITC-albumin on the apical side for permeability measurements (right panel). Data are represented as mean+/−SEM of 3–5 inserts/per condition, n = 3. (B) TEM images of crosscuts obtained from untreated (-) and LT-treated lung epithelial layers. Crosscuts with ciliated cells on the apical side are depicted. Scale bar represents 10 µm. (C) Confocal images of the apical side of untreated and LT-treated lung epithelial layers using a tight junction marker (ZO1, green). Z-series was analyzed with Imaris5 (Bitplane Inc., MN). Disruptions of multicellular junctions are indicated by white arrows. The scale bar represents 50 µm. (D) LDH release of untreated and LT-treated NHBE 3D layers. Data are represented as mean+/−SEM of 3 inserts, n = 3.