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. 2000 Feb 15;97(4):1677–1682. doi: 10.1073/pnas.97.4.1677

Figure 2.

Figure 2

Expression of CA VB in COS-7 cells and different mouse tissues. (A) The cell homogenate or cell mitochondria from COS-7 cells transfected with vector only, CA VA and CAVB cDNAs clone nos. 19 and 21 were analyzed by SDS/PAGE followed by immunoblotting. The polypeptides for CA VA and CA VB are marked (Fig. 1). (B) Homogenates of different tissues containing 50 μg of protein were analyzed by SDS/PAGE followed by immunoblotting by using anti-mouse CA VB C-tail or CA VA C-tail antisera. Lanes: 1, brain; 2, heart; 3, liver; 4, lung; 5, kidney; 6, spleen; 7, intestine; 8, testis; 9, muscle; 10, pancreas. The polypeptides of 31-kDa mature CA VB were seen in all tissues used here. The polypeptides of 31-kDa mature CA VA and proteolytically nicked 28-kDa polypeptides were seen in few tissues.