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Carbonic anhydrase activity in cell homogenate and mitochondria of COS-7 cells transfected with CA VB
| Enzyme | CA activity, enzyme units/mg protein*
|
|
|---|---|---|
| Cell homogenate | Mitochondria | |
| Vector only | 0.09 | 0.01 |
| mCA VA | 0.48 | 3.65 |
| mCA VB no. 19 | 0.46 | 3.80 |
| mCA VB no. 21 | 0.43 | 3.80 |
mCA VB cDNAs from two independent clones nos. 19 and 21 were used; both expressed CA activity.
*Average of two measurements.
